Molecular basis of basal cell carcinoma: analysis of differential gene expression by differential display PCR and expression array.
Identifieur interne : 000F25 ( Main/Exploration ); précédent : 000F24; suivant : 000F26Molecular basis of basal cell carcinoma: analysis of differential gene expression by differential display PCR and expression array.
Auteurs : Thomas Welss [Allemagne] ; Marina Papoutsaki ; Günter Michel ; Julia Reifenberger ; Sergio Chimenti ; Thomas Ruzicka ; Harry F. AbtsSource :
- International journal of cancer [ 0020-7136 ] ; 2003.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ARN messager (biosynthèse), ARN messager (génétique), ARN tumoral (biosynthèse), ARN tumoral (génétique), Analyse de profil d'expression de gènes (MeSH), Banque de gènes (MeSH), Carcinome basocellulaire (génétique), Humains (MeSH), Kératinocytes (métabolisme), Lignée cellulaire (métabolisme), Lignée de cellules transformées (métabolisme), Protéines tumorales (biosynthèse), Protéines tumorales (génétique), Réaction de polymérisation en chaîne (méthodes), Régulation de l'expression des gènes tumoraux (MeSH), Séquençage par oligonucléotides en batterie (MeSH), Technique de soustraction (MeSH), Tumeurs cutanées (génétique).
- MESH :
- biosynthèse : ARN messager, ARN tumoral, Protéines tumorales.
- génétique : ADN complémentaire, ARN messager, ARN tumoral, Carcinome basocellulaire, Protéines tumorales, Tumeurs cutanées.
- métabolisme : Kératinocytes, Lignée cellulaire, Lignée de cellules transformées.
- méthodes : Réaction de polymérisation en chaîne.
- Analyse de profil d'expression de gènes, Banque de gènes, Humains, Régulation de l'expression des gènes tumoraux, Séquençage par oligonucléotides en batterie, Technique de soustraction.
English descriptors
- KwdEn :
- Carcinoma, Basal Cell (genetics), Cell Line (metabolism), Cell Line, Transformed (metabolism), DNA, Complementary (genetics), Gene Expression Profiling (MeSH), Gene Expression Regulation, Neoplastic (MeSH), Gene Library (MeSH), Humans (MeSH), Keratinocytes (metabolism), Neoplasm Proteins (biosynthesis), Neoplasm Proteins (genetics), Oligonucleotide Array Sequence Analysis (MeSH), Polymerase Chain Reaction (methods), RNA, Messenger (biosynthesis), RNA, Messenger (genetics), RNA, Neoplasm (biosynthesis), RNA, Neoplasm (genetics), Skin Neoplasms (genetics), Subtraction Technique (MeSH).
- MESH :
- chemical , biosynthesis : Neoplasm Proteins, RNA, Messenger, RNA, Neoplasm.
- chemical , genetics : DNA, Complementary, Neoplasm Proteins, RNA, Messenger, RNA, Neoplasm.
- genetics : Carcinoma, Basal Cell, Skin Neoplasms.
- metabolism : Cell Line, Cell Line, Transformed, Keratinocytes.
- methods : Polymerase Chain Reaction.
- Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Gene Library, Humans, Oligonucleotide Array Sequence Analysis, Subtraction Technique.
Abstract
Basal cell carcinoma (BCC) is the most common tumor in the Caucasian population. Although BCC rarely metastasize and cause death, they are problematic due to their destructive growth and the frequent localization on the face. Until now the knowledge of genes differentially expressed in BCC has been incomplete. To elucidate the complex alterations in BCC-associated gene expression, we took advantage of 2 techniques: the differential display RT-PCR (DD-PCR) and the differential hybridization of cDNA arrays. Using DD-PCR, we showed differential expression of genes known from other biological contexts (e.g., rac, ubiquitin hydrolase), which could now be associated with BCC. In addition, we detected unknown genes possibly contributing to the carcinogenesis of BCC. Of the 588 genes screened by differential hybridization of the Atlas human cDNA array, differences in the expression levels of BCC were observed for 10 genes. These data were obtained with RNA probes pooled from several BCC of different donors and were subsequently confirmed by semiquantitative RT-PCR for Janus protein tyrosine kinase 3 (Jak3), microsomal glutathione S-transferase 1 (GST 12), teratocarcinoma-derived growth factor cripto, glutaredoxin and the monocyte chemoattractant protein 1 (MCP-1) in 10 individual BCC specimens, 2 squamous cell carcinoma (SCC), the cell line HaCaT and cultured normal human keratinocytes (NHK) in comparison to normal skin. These genes are candidates from gene families with known association to tumors, but they have not been reported in the carcinogenesis of BCC yet. In summary, both approaches allow the detection of differentially expressed genes possibly involved in the carcinogenesis of BCC.
DOI: 10.1002/ijc.10912
PubMed: 12532421
Affiliations:
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Thomas.Welss@Henkel.com</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Dermatology, Heinrich-Heine University Düsseldorf, Düsseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Papoutsaki, Marina" sort="Papoutsaki, Marina" uniqKey="Papoutsaki M" first="Marina" last="Papoutsaki">Marina Papoutsaki</name></author><author><name sortKey="Michel, Gunter" sort="Michel, Gunter" uniqKey="Michel G" first="Günter" last="Michel">Günter Michel</name></author><author><name sortKey="Reifenberger, Julia" sort="Reifenberger, Julia" uniqKey="Reifenberger J" first="Julia" last="Reifenberger">Julia Reifenberger</name></author><author><name sortKey="Chimenti, Sergio" sort="Chimenti, Sergio" uniqKey="Chimenti S" first="Sergio" last="Chimenti">Sergio Chimenti</name></author><author><name sortKey="Ruzicka, Thomas" sort="Ruzicka, Thomas" uniqKey="Ruzicka T" first="Thomas" last="Ruzicka">Thomas Ruzicka</name></author><author><name sortKey="Abts, Harry F" sort="Abts, Harry F" uniqKey="Abts H" first="Harry F" last="Abts">Harry F. 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Thomas.Welss@Henkel.com</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Dermatology, Heinrich-Heine University Düsseldorf, Düsseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Papoutsaki, Marina" sort="Papoutsaki, Marina" uniqKey="Papoutsaki M" first="Marina" last="Papoutsaki">Marina Papoutsaki</name></author><author><name sortKey="Michel, Gunter" sort="Michel, Gunter" uniqKey="Michel G" first="Günter" last="Michel">Günter Michel</name></author><author><name sortKey="Reifenberger, Julia" sort="Reifenberger, Julia" uniqKey="Reifenberger J" first="Julia" last="Reifenberger">Julia Reifenberger</name></author><author><name sortKey="Chimenti, Sergio" sort="Chimenti, Sergio" uniqKey="Chimenti S" first="Sergio" last="Chimenti">Sergio Chimenti</name></author><author><name sortKey="Ruzicka, Thomas" sort="Ruzicka, Thomas" uniqKey="Ruzicka T" first="Thomas" last="Ruzicka">Thomas Ruzicka</name></author><author><name sortKey="Abts, Harry F" sort="Abts, Harry F" uniqKey="Abts H" first="Harry F" last="Abts">Harry F. Abts</name></author></analytic><series><title level="j">International journal of cancer</title><idno type="ISSN">0020-7136</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carcinoma, Basal Cell (genetics)</term><term>Cell Line (metabolism)</term><term>Cell Line, Transformed (metabolism)</term><term>DNA, Complementary (genetics)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Neoplastic (MeSH)</term><term>Gene Library (MeSH)</term><term>Humans (MeSH)</term><term>Keratinocytes (metabolism)</term><term>Neoplasm Proteins (biosynthesis)</term><term>Neoplasm Proteins (genetics)</term><term>Oligonucleotide Array Sequence Analysis (MeSH)</term><term>Polymerase Chain Reaction (methods)</term><term>RNA, Messenger (biosynthesis)</term><term>RNA, Messenger (genetics)</term><term>RNA, Neoplasm (biosynthesis)</term><term>RNA, Neoplasm (genetics)</term><term>Skin Neoplasms (genetics)</term><term>Subtraction Technique (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>ARN messager (biosynthèse)</term><term>ARN messager (génétique)</term><term>ARN tumoral (biosynthèse)</term><term>ARN tumoral (génétique)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Banque de gènes (MeSH)</term><term>Carcinome basocellulaire (génétique)</term><term>Humains (MeSH)</term><term>Kératinocytes (métabolisme)</term><term>Lignée cellulaire (métabolisme)</term><term>Lignée de cellules transformées (métabolisme)</term><term>Protéines tumorales (biosynthèse)</term><term>Protéines tumorales (génétique)</term><term>Réaction de polymérisation en chaîne (méthodes)</term><term>Régulation de l'expression des gènes tumoraux (MeSH)</term><term>Séquençage par oligonucléotides en batterie (MeSH)</term><term>Technique de soustraction (MeSH)</term><term>Tumeurs cutanées (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Neoplasm Proteins</term><term>RNA, Messenger</term><term>RNA, Neoplasm</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>Neoplasm Proteins</term><term>RNA, Messenger</term><term>RNA, Neoplasm</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ARN messager</term><term>ARN tumoral</term><term>Protéines tumorales</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Carcinoma, Basal Cell</term><term>Skin Neoplasms</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>ARN messager</term><term>ARN tumoral</term><term>Carcinome basocellulaire</term><term>Protéines tumorales</term><term>Tumeurs cutanées</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Line</term><term>Cell Line, Transformed</term><term>Keratinocytes</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Kératinocytes</term><term>Lignée cellulaire</term><term>Lignée de cellules transformées</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Réaction de polymérisation en chaîne</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Neoplastic</term><term>Gene Library</term><term>Humans</term><term>Oligonucleotide Array Sequence Analysis</term><term>Subtraction Technique</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Banque de gènes</term><term>Humains</term><term>Régulation de l'expression des gènes tumoraux</term><term>Séquençage par oligonucléotides en batterie</term><term>Technique de soustraction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Basal cell carcinoma (BCC) is the most common tumor in the Caucasian population. Although BCC rarely metastasize and cause death, they are problematic due to their destructive growth and the frequent localization on the face. Until now the knowledge of genes differentially expressed in BCC has been incomplete. To elucidate the complex alterations in BCC-associated gene expression, we took advantage of 2 techniques: the differential display RT-PCR (DD-PCR) and the differential hybridization of cDNA arrays. Using DD-PCR, we showed differential expression of genes known from other biological contexts (e.g., rac, ubiquitin hydrolase), which could now be associated with BCC. In addition, we detected unknown genes possibly contributing to the carcinogenesis of BCC. Of the 588 genes screened by differential hybridization of the Atlas human cDNA array, differences in the expression levels of BCC were observed for 10 genes. These data were obtained with RNA probes pooled from several BCC of different donors and were subsequently confirmed by semiquantitative RT-PCR for Janus protein tyrosine kinase 3 (Jak3), microsomal glutathione S-transferase 1 (GST 12), teratocarcinoma-derived growth factor cripto, glutaredoxin and the monocyte chemoattractant protein 1 (MCP-1) in 10 individual BCC specimens, 2 squamous cell carcinoma (SCC), the cell line HaCaT and cultured normal human keratinocytes (NHK) in comparison to normal skin. These genes are candidates from gene families with known association to tumors, but they have not been reported in the carcinogenesis of BCC yet. In summary, both approaches allow the detection of differentially expressed genes possibly involved in the carcinogenesis of BCC.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12532421</PMID><DateCompleted><Year>2003</Year><Month>03</Month><Day>25</Day></DateCompleted><DateRevised><Year>2016</Year><Month>03</Month><Day>03</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0020-7136</ISSN><JournalIssue CitedMedium="Print"><Volume>104</Volume><Issue>1</Issue><PubDate><Year>2003</Year><Month>Mar</Month><Day>10</Day></PubDate></JournalIssue><Title>International journal of cancer</Title><ISOAbbreviation>Int J Cancer</ISOAbbreviation></Journal><ArticleTitle>Molecular basis of basal cell carcinoma: analysis of differential gene expression by differential display PCR and expression array.</ArticleTitle><Pagination><MedlinePgn>66-72</MedlinePgn></Pagination><Abstract><AbstractText>Basal cell carcinoma (BCC) is the most common tumor in the Caucasian population. Although BCC rarely metastasize and cause death, they are problematic due to their destructive growth and the frequent localization on the face. Until now the knowledge of genes differentially expressed in BCC has been incomplete. To elucidate the complex alterations in BCC-associated gene expression, we took advantage of 2 techniques: the differential display RT-PCR (DD-PCR) and the differential hybridization of cDNA arrays. Using DD-PCR, we showed differential expression of genes known from other biological contexts (e.g., rac, ubiquitin hydrolase), which could now be associated with BCC. In addition, we detected unknown genes possibly contributing to the carcinogenesis of BCC. Of the 588 genes screened by differential hybridization of the Atlas human cDNA array, differences in the expression levels of BCC were observed for 10 genes. These data were obtained with RNA probes pooled from several BCC of different donors and were subsequently confirmed by semiquantitative RT-PCR for Janus protein tyrosine kinase 3 (Jak3), microsomal glutathione S-transferase 1 (GST 12), teratocarcinoma-derived growth factor cripto, glutaredoxin and the monocyte chemoattractant protein 1 (MCP-1) in 10 individual BCC specimens, 2 squamous cell carcinoma (SCC), the cell line HaCaT and cultured normal human keratinocytes (NHK) in comparison to normal skin. These genes are candidates from gene families with known association to tumors, but they have not been reported in the carcinogenesis of BCC yet. In summary, both approaches allow the detection of differentially expressed genes possibly involved in the carcinogenesis of BCC.</AbstractText><CopyrightInformation>Copyright 2002 Wiley-Liss, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Welss</LastName><ForeName>Thomas</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Dermatology, Heinrich-Heine University Düsseldorf, Düsseldorf, Germany. Thomas.Welss@Henkel.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Papoutsaki</LastName><ForeName>Marina</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Michel</LastName><ForeName>Günter</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Reifenberger</LastName><ForeName>Julia</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Chimenti</LastName><ForeName>Sergio</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Ruzicka</LastName><ForeName>Thomas</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Abts</LastName><ForeName>Harry F</ForeName><Initials>HF</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Int J Cancer</MedlineTA><NlmUniqueID>0042124</NlmUniqueID><ISSNLinking>0020-7136</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009363">Neoplasm Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012334">RNA, Neoplasm</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002280" MajorTopicYN="N">Carcinoma, Basal Cell</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002461" MajorTopicYN="N">Cell Line, Transformed</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="Y">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015972" MajorTopicYN="Y">Gene Expression Regulation, Neoplastic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015723" MajorTopicYN="N">Gene Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015603" MajorTopicYN="N">Keratinocytes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009363" MajorTopicYN="N">Neoplasm Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020411" MajorTopicYN="Y">Oligonucleotide Array Sequence Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012334" MajorTopicYN="N">RNA, Neoplasm</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012878" MajorTopicYN="N">Skin Neoplasms</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013382" MajorTopicYN="N">Subtraction Technique</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>1</Month><Day>18</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>3</Month><Day>26</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>1</Month><Day>18</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12532421</ArticleId><ArticleId IdType="doi">10.1002/ijc.10912</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>District de Düsseldorf</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Düsseldorf</li></settlement></list><tree><noCountry><name sortKey="Abts, Harry F" sort="Abts, Harry F" uniqKey="Abts H" first="Harry F" last="Abts">Harry F. Abts</name><name sortKey="Chimenti, Sergio" sort="Chimenti, Sergio" uniqKey="Chimenti S" first="Sergio" last="Chimenti">Sergio Chimenti</name><name sortKey="Michel, Gunter" sort="Michel, Gunter" uniqKey="Michel G" first="Günter" last="Michel">Günter Michel</name><name sortKey="Papoutsaki, Marina" sort="Papoutsaki, Marina" uniqKey="Papoutsaki M" first="Marina" last="Papoutsaki">Marina Papoutsaki</name><name sortKey="Reifenberger, Julia" sort="Reifenberger, Julia" uniqKey="Reifenberger J" first="Julia" last="Reifenberger">Julia Reifenberger</name><name sortKey="Ruzicka, Thomas" sort="Ruzicka, Thomas" uniqKey="Ruzicka T" first="Thomas" last="Ruzicka">Thomas Ruzicka</name></noCountry><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Welss, Thomas" sort="Welss, Thomas" uniqKey="Welss T" first="Thomas" last="Welss">Thomas Welss</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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